Information-enriched characterization of mobile populations. Morphological details from photos can incorporate into the richness of flow cytometry knowledge. For example, the figure displays an usually regular apoptosis assay employing Annexin V and PI, adding mobile imaging to characterize cells in each population to reveal morphologically distinctive functions. These insights could not are already obtained from multiplex staining by yourself.
As samples are acquired on the Attune CytPix Flow Cytometer, the large-speed brightfield camera captures and shops visuals of detected events in a fee nearly six,000 pictures for every second, depending on the flow level and picture sizing. For higher overall flexibility, Attune Cytometric Software package permits you to adjust the picture capture frequency as necessary and choose a particular gate for picture capture, if necessary.
Blend acoustic focusing technological know-how with superior-parameter analysis for versatile spectral unmixing and conventional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating having a substantial-pace brightfield digital camera for simultaneous higher throughput flow cytometry and higher resolution brightfield imaging.
"An image isn't really rather worth one thousand dots, but it surely genuinely helps you to see illustrations or photos. It is excellent to find out images that correlate with staining. By way of example, AnnexinV-constructive cells which can be morphologically various than healthy cells, or CD14+ monocytes which are greater plus more textured than CD3+/CD4+/CD14- lymphocytes.
Moreover, it is illuminating to see the number of doublets slip by conventional singlet gates, and great to verify the standing of unanticipated double-good situations in my analysis."
Pair your Attune flow cytometer by having an autosampler for increased workflow effectiveness, and increase robotic integration for max throughput.
Regular picture quality even at significant throughput Acoustic concentrating along with a high-velocity camera Mix to image these T cells continually at reduced or higher flow rates. Quickly alter target and digital camera configurations to fulfill experimental necessities.
The Attune NxT cytometer is definitely the reliable workhorse instrument your lab can trust in for constant and dependable performance. The Attune CytPix design encompasses a high-pace brightfield camera and AI-driven picture Examination computer software, with latest upgrades such as machine Finding out resources and an intuitive person interface, enabling enhanced details precision, precision, and more rapidly insights into sample biology.
Maybe most significantly, these functions incorporate cells of Evidently various phenotypes which may lead to incorrect conclusions regarding double good occasions (especially in scarce populations).
AI-pushed automated graphic analysis with the Attune Cytometric Software program interprets occasion attributes into distinctive morphology parameters that may be combined with common fluorescence and scatter parameters to expose remarkably reproducible populace adjustments and distinctive subsets.
We Beforehand shown the strength of imaging Vehicle-T/Ramos cell interactions. Let’s check out just the populace of greatest curiosity, the double beneficial events, To find out more. We are able to now use prolonged morphological parameters (here, circularity and skewness of depth) to further study the capabilities of these populations and refine gating on these situations, raising information robustness.
Leverage the strength of State-of-the-art technological innovation and innovation to speed up check here your discoveries. Trust within the Attune Flow Cytometer relatives to deliver the trustworthiness, velocity, and accuracy that set your perform apart.
No clean/no lyse leukocyte Investigation with imaging. Cells were acquired from 24-hour-aged blood by dilution in one mM EDTA ( Expert UX/UI allows users to practice AI types for expanded applications. (A) The “Prepare Model” element provides annotation applications and genuine-time design teaching precision.
At the guts of each and every Attune Flow Cytometer lies our revolutionary acoustic focusing technological innovation, which makes use of seem waves to meticulously align cells in the flow mobile. This innovative tactic surpasses regular hydrodynamic solutions, causing more quickly processing speeds, exceptional details quality, and improved sample throughput.
The Attune Xenith cytometer is actually a substantial-parameter spectral analyzer, giving equally spectral unmixing and standard flow abilities for in depth and functional mobile Assessment.
The Attune CytPix Flow Cytometer lets people to visualise samples through acquisition, validate the precision of gating methods, and help results with trusted documentation for publication functions.
Here, we show that by gating procedures applying picture-dependent quantitative parameters can distinguish interacting cells from coincident situations to extra correctly review interacting cells.